Check For Kdr Mutations (1016 And 1534) In Different Field Mosquito Populations Of Aedes Aegypti In Brazil

Several mutations in voltage gated sodium channel (NAv) have been identified in Aedes aegypti populations
worldwide. The variations in the V1016I and F1534C are the most related to knockdown resistance to pyrethroids. A study was carried out to determine the frequency of V1016I and F1534C NAv by real-time PCR in four natural populations of Ae. aegypti of different region of Brazil during 2021 and compared with susceptible population (Rockefeller strain) of Ae. aegypti. In natural populations, average frequencies of V1016I varied between to 30.5 and 55% were found and frequencies of F1534C between 53.8 and 100% comparing with the susceptible strain that presented no frequencies to V1016I and F1534C. The results showed that F1534C frequencies are higher than V1016I frequencies in natural populations of Ae. aegypti in Brazil providing useful information for future control.