Species Identification Of The Anopheles Fluviatilis Complex (Diptera: Culicidae) Using Phylogenetic Analysis Pcr-Sequencing In Southwestern Iran

Anopheles fluviatilis is one of the most important malaria vectors in Iran. The aim of this study was to identify Anopheles fluviatilis complex species using proliferation and sequence analysis of ITS2 and also 28.S- D3parts of rDNA gene in southwestern Iran. In this research Anopheles fluviatilis was caught from different areas of Kohgiluyeh and Boyer-Ahmad province at southwestern Iran in 2013. DNA was taken from 4 Anopheles fluviatilis selected samples and PCR tests of 28S- D3 part were done on these DNA samples. Then after the sequence results were obtained, these were identified and compared with similar samples of Anopheles fluviatilis based on data from a gene word bank. Phylogenetic tree and individual sequences of samples were calculated. PCR Phylogenetic analysis of r DNA 28S-D3 part of Anopheles fluviatilis confirmed that U genotype in Kohgiluyeh and Boyer-Ahmad province was identified. The gene length of r DNA 28S-D3 part between populations of this species was the same (333bp). This study showed that Anopheles fluviatilis has a species separate branch in southwestern Iran which is different to the branch of southeastern Iran (Hormozgan, Kerman and Sistaan & Baluchestaan provinces).