Mosquito-Based Arboviral Survey In Western Lombardy, Italy: 2009–2013
  Key Words  
  Arbovirus, surveillance, Culex pipiens, Anopheles maculipennis, Ochlerotatus caspius, Usutu virus, Tahyna virus, Batai virus
  Mattia Calzolari, Francesco Defilippo, Marco Caimi, Sara Savoldelli, Paolo Fedeli, Valentina Parco, Giuseppe Braghieri, Massimo Fabbi, Giorgio Varisco, Stefano Cinotti, And Michele Dottori
  A mosquito-based arbovirus surveillance was settled in “Parco Lombardo delle Valli del Ticino” and in neighbouring sites in Milano province (Lombardy). Nine sites were monitored between 2009 and 2013 sampling 82,106 mosquitoes, of which 68.6 % belonged to Culex pipiens L. species. Mosquito were sorted in pools according to species, data and place of collection and submitted to biomolecular analysis for detection of flaviviruses and orthobunyaviruses. Usutu virus was detected in three different pools of Cx. pipiens, Batai virus was detected in a pool of Anopheles maculipennis s. l. and in two pools of Cx. pipiens, and Tahyna virus in a pool of Aedes vexans (Meigen) and in one of Ochlerotatus caspius (Pallas). Obtained data give useful information on mosquito community in surveyed area and demonstrate the ability of this surveillance system to detect arboviruses circulating in mosquitoes.